[DGIST 관련자료] DGIST 김민수-구재형 교수 연구 성과 요약본.hwp

DGIST - MRPrimer: A MapReduce-based method for the thorough design of valid and ranked primers for PCR Hyerin Kim*, NaNa Kang*, Kang-Wook Chon, Seonho Kim, NaHye Lee, JaeHyung Koo+, and Min-Soo Kim+ (Nucleic Acids Research, online publication, IF=9.112) *; equal contribution, +; co-corresponding author ABSTRACT Primer design is a fundamental technique that is widely used for polymerase chain reaction (PCR). Although many methods have been proposed for primer design, they require a great deal of manual effort to generate feasible and valid primers, including homology tests on off-target sequences using BLAST-like tools. That approach is inconvenient for many target sequences of quantitative PCR (qPCR) due to considering the same stringent and allele-invariant constraints. To address this issue, we propose an entirely new method called MRPrimer that can design all feasible and valid primer pairs existing in a DNA database at once, while simultaneously checking a multitude of filtering constraints and validating primer specificity. Furthermore, MRPrimer suggests the best primer pair for each target sequence, based on a ranking method. Through qPCR analysis using 343 primer pairs and the corresponding sequencing and comparative analyses, we showed that the primer pairs designed by MRPrimer are very stable and effective for qPCR. In addition, MRPrimer is computationally efficient and scalable, and therefore useful for quickly constructing an entire collection of feasible and valid primers for frequently updated databases like RefSeq. Furthermore, we suggest that MRPrimer can be utilized conveniently for experiments requiring primer design, especially real-time qPCR. (MERS) (Ebola) (SARS) (PCR) . , . , . PCR (Primer) . () 2 3 3 3 , . qPCR . Howard Hughes Medical Institute (NIH, National Institutes of Health) Primer3 Primer-BLAST . , Primer3 1 3 10 . Primer3 , BLAST , . , . (de-facto standard) Primer3+BLAST 10 DGIST (MRPrimer) . () (MapReduce) . , . MRPrimer . 4 MRPrimer Nucleic Acids Research(Oxford Press ) 24() , . MRPrimer , MRPrimer SW .(http://MRPrimer.com ) MRPrimer MRPrimer . , , . Primer3 + BLAST , . , MRPrimer . , GMO( ) , . MRPrimer . (http://MRPrimer.com) MRPrimer SW . , MRPrimer S/W 10 . ? . , MRPrimer SW . . . 3% 1,000 , . Primer3+BLAST . . , 3 . . , MRPrimer . . . MRPrimer . Primer3 1 3 . MRPrimer , ( MRPrimer+). , . MRPrimer . 7 , . ( ) . 7 , (Map) (Reduce) . 1: . 2: 1 (: ) . . 3: . . 4: 3 k . . 5: 4 (false positive) . k 1 4-5 . k 1, 2~3 4-5 . 6: 5 . . 7: 6 , . .

2024-02-28

0920 오세정 한국연구재단 이사장 초청_제3회 리더십 강좌 개최.hwp

2011. 09. 20() 053-785-1132, 011-9588-2809, fax 053-785-1109 [] 053-785-5110, 011-9578-5413 - 3 DGIST - DGIST(, ) 21() 4 30 . DGIST 3 DGIST , 100 , 21 ' . 5 DGIST DGIST . 1975 , 1981 , , , . . , , , , . : 1. 1 2. 1() 3. 3 DGIST 1(). . 1. ( ) 1975 1981 1981~1984 Xerox Palo Alto 1984~ 1987~1995 International Center for Theoretical Physics Associate Member 1989~1994 21 1994~1994 The University of Tokyo Visiting Professor 1999~2001 5 1999~2008 2000~ 2003~2003 2004~2005 2 BK21 2004~2006 8, 9 2004~2008 2007~2009 2008~2009 2008~2009 1 2009~ 2009~ , Fellow 2011~ 2011~ 2011~ (1994) (1998) (2003)

2024-02-28

한국연구재단) 2023넌 대학 정부연구비 관리 우수 사례. 수기 공모전 공고.pdf

1 2023 (23. 9.27. ) 1. 2. () 2023 () () / ( ) [1] A4 2 ( ) 5 (, ), [2] A4 2 5 (2023. 9.27.) . () , / () , () '23.10.16.() ~ '23.10.25.() 17:00 () ( ) ( ) (rnd@ nrf.re .kr) ( ) 5 ( ) (4 ) mailto:rnd@nrf.re.kr 2 () , . : 5 ? 25 ? 25 , ? 25 , ? 25 100 ( ) . , , 5 5 (3) 1 1 1 150 100 (6) 2 2 2 100 50 9 ( ) '23. 11 () ( ) 3 , , . , , 2 . , , , . , , . , , . . , , . 2023 () (24. 1 ) . ( 042-869-6645)

2023-10-04

붙임2. 한국연구재단 지원 계속과제의 연구수당 가이드라인 Q&A.hwp

Q&A . (https://nrf.re.kr) Q&A 1. ? - 2022 3 . 2021. 6.30. 2021. 6.30. 2021. 1. 1. 2 , (2021.1.1.) 1 2. , 0 ? - (0 ) . () . 3. 2021.6.30. 2022.1.1. . ? - . 2021.6.30. . 4. 2021.6.30. , 20% . ? - . , . , 2021.6.30. . 5. 2021.6.30. , 2022 3 . ? - . . 2021. 6.30. . , [ ] . , , , , , , * , () () * () 2021.6.30 ( ) ( ( )) 1~5 6 (6 ) ( ) 2022 6 (2017 ) 2022 2023 6 (2018 ) 2023 6 * 2022 5 * * ( ) 1 2~3 (1 ) 2020 2020 2017~2019 2022 * 2012~2016 ( ) 2022 2022 ** * ** , , ( ) - : , 2021 ( ) ( ) - 2017~2020 2023 * * < > 110 (11) ) 1 2 10 11 (n) (n+1) (n+2) (n+10) (n+11) ( ) 2021.6.30. < () > 2017 2018 2019 2020 2021* 2022 2023 2024.. 2017 1 2 3 4 5 ( ) 6 ** - - 2018 - 1 2 3 4 5 ( ) 6 ** - 2019 - - 1 2 3 4 5 - 2020 3 2020 6 - - - 1 2 3 4 5 2020 9 - - - 1 2 *** 3 4 5 2021 - - - - 1 2 3 4 2022 - - - - - 1 2 3 * 2021.6.30. 2021 ** 1~5 , 6 (2018 6(2023) 2022 ) *** 2021.6.30. 2021 2021 < () > 2023 1 2016 2017 2018 2019 2020 2021 2022 2023 2016 1 2 3 4 5 6 1 7 - 2017 - 1 2 3 4 5 1 6 1 7 2018 - - 1 2 3 4 1 5 1 6 2019 - - - 1 2 3 1 4 1 5 2020 - - - - 1 2 1 3 1 4 2021 - - - - - 1 1 2 1 3 2022 - - - - - - 1 1 1 2 < () 2019 > 2021.6.30. () , - 2019~2021 , 2022 2019 2020 2021 2022 2023 () (2020 ) - - 1 2 2 2 2 200 200 200 200 200 1 - 400 - - - - - - 400* 400 400 - 200 400 400 200 200 14 * 2021.6.30. ( 2021.6.30. 2021 2021 400 200 )

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